Journal: Antioxidants

Article Title: Olive Leaf Extract Added to Losartan Treatment Improved Klotho/Wnt/β-Catenin Signaling in Hypertensive Rats with Focal Segmental Glomerulosclerosis

doi: 10.3390/antiox15010146

Figure Lengend Snippet: Effects of L, L+T and L+O on 6–nitrotryptophan, Klotho, Wnt4, β–catenin, PAI–1 and fibronectin protein expressions in the kidney of SHR with ADR-induced FSGS. The analysis of 6–nitrotryptophan/actin ( A ), Klotho/actin ( B ), Wnt4/GAPDH ( C ), β–catenin/GAPDH ( D ), PAI–1/GAPDH ( E ), fibronectin/GAPDH ( F ) protein expressions and representative Western blots ( G ). Values represent mean ± SEM of three to four independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. SHC; # p < 0.05 and ### p < 0.001 vs. SHADR; $$ p < 0.01 and $$$ p < 0.001 vs. SHADR+L; and &&& p < 0.001 vs. SHADR+L+T. SHC, control group; SHADR, model group; L, losartan; T, tempol; and O, olive leaf extract.

Article Snippet: The membranes were blocked with 5% non-fat dry milk in Tris-buffered saline with 0.1% Tween 20 (TBS-Tween) and then incubated with the following primary antibodies: 6-nitrotryptophan (1:1000, ab243072, Abcam, Cambridge, UK), Klotho (1:1000, NBP1-76511, Novus biologicals, LCC, USA), Wnt-4 (1:2000, NBP2-20909, Novus biologicals, LCC, Centennial, CO, USA), β-catenin (1:300, AF1329, R&D Systems, Inc., Minneapolis, MN, USA), PAI-1 (1:1000, NBP1-19773, Novus biologicals, LCC, Centennial, CO, USA), fibronectin (1:1000, ab2413, Abcam, Cambridge, UK), actin (1:500, A5060, Sigma-Aldrich, MO, USA) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2500, ab9485, Abcam, Cambridge, UK).

Techniques: Western Blot, Control